Fig. 4

High-resolution melting curve analysis of putative DNA-repair genes in maize: ZeaRAD51 and ZeaBRCA1. Melting curves were created as a negative first derivative (−d(RFU)/d(T)) using non-normalized relative fluorescence values (RFUs) for RAD51 (A) and BRCA1 (D). Difference curves are shown for inbred lines (B73 or Mo17) against purple landraces (P1 and P2) for RAD51 (B and C, respectively) and BRCA1 (E and F, respectively) (n = 3, 3 biological samples and 3 technical replicates)