Fig. 3

High-resolution melting curve analysis of putative DNA-repair genes in maize: ZeaATM1 and ZeaSOG1. Melting curves were created as a negative first derivative (− d(RFU)/d(T)) using non-normalized relative fluorescence values (RFUs) for ATM1 (A) and SOG1 (D). Difference curves are shown for inbred lines (B73 or Mo17) against purple landraces (P1 and P2) for ATM1 (B and C, respectively) and SOG1 (E and F, respectively) (n = 3, 3 biological samples and 3 technical replicates)